Intersectin is a scaffolds,protein that functions as a master regulator of endocytosis and signaling. It contains multiple protein interaction domains and links various components of the endocytic machinery.
[^1]
| Property | Value | [^2]
|----------|-------| [^3]
| **Protein Name** | Intersectin | [^4]
| **Gene** | ITSN1 (Intersectin 1), ITSN2 (Intersectin 2) |
| **UniProt ID** | [Q15811](https://www.uniprot.org/uniprot/Q15811) (ITSN1) |
| **Molecular Weight** | ~200 kDa (ITSN1), ~172 kDa (ITSN2) |
| **Subcellular Localization** | Cytoplasm, plasma membrane, clathrin-coated pits |
| **Protein Family** | EHS1/Intersectin family |
¶ Domain Organization
- N-terminal EH domain: Binds to NPF motifs in endocytic proteins
- Coiled-coil regions: Dimerization and protein interactions
- SH3 domains (x5): Bind to proline-rich motifs in dynamin, synaptojanin, N-WASP
- C-terminal SH3 domains: Additional binding sites
- Clathrin coat organization: Recruits clathrin and adaptors
- Dynamin binding: SH3 domains bind dynamin GTPase
- Synaptojanin recruitment: Links to phosphoinositide metabolism
- Actin regulation: Activates N-WASP for actin polymerization
- Nerve growth factor signaling: Adaptor for Trk receptors
- Ras signaling: Can activate SOS and Ras pathways
- Synaptic plasticity: Regulates spine morphology
- Vesicle formation: Coordinates early endocytic events
- Endophilin recruitment: Bridges to actin dynamics
- Fission regulation: Modulates dynamin activity
- Alzheimer's disease: Links to APP processing and Aβ production
- Parkinson's disease: Regulates synaptic vesicle recycling
- Huntington's disease: Altered expression in HD
- Oncogenic signaling: Overexpression in various cancers
- Cell transformation: Can activate Ras/MAPK pathway